CHRONIC MYELOID LEUKAEMIA (CML): BCR-ABL DIAGNOSTIC SCREEN & MONITORING

The t(9;22) translocation results in the formation of the BCR-ABL transcript which can be detected, using PCR, in approximately 95% of cases of Chronic Myeloid Leukaemia, 25% of adult cases of Acute Lymphoblastic Leukaemia (ALL), 5% of childhood cases of ALL and 1% of cases of Acute Myeloid Leukaemia (AML). The PCR uses primers described by Cross et al. 1994 Leukemia 8:186-9. BCR-ABL transcript levels can be quantified by Real-Time PCR to monitor response to treatment.

Laboratory contact: Dr Caroline Wickham 01392 408252 caroline.wickham@nhs.net

Clinical contact: Dr Paul Kerr 01392 402917 paul.kerr1@nhs.net

The laboratory participates in the NEQAS LI BCR-ABL and AML Translocation Identification Programme and the NEQAS LI  BCR-ABL Quantitation EQA scheme