Cerebrospinal Fluid (CSF) is located within the subarachnoid space. It is produced by the choroids plexuses and drains into the venous sinuses. Approximately 100mls of CSF is in continuous circulation. CSF may reflect pathology within the brain, spinal cord or membranes. CSF cytology is indicated as part of an investigation of known or suspected CNS malignancy (primary or secondary) or in cases of aseptic meningitis.
CSF must not be submitted for cytological examination where the clinical diagnosis is of known or suspected CJD. In such cases, there should be prior discussion with a consultant cytopathologist.
In accordance with Laboratory Safety Committee guidelines, acellular or paucicellular specimens may not be processed by the Cytopathology Department. In this case, we will issue a report referring to the microbiological result only.
CSF cytology is usually performed in conjunction with CSF microbiology and biochemistry tests. CSF cytology is indicated as part of an investigation of known or suspected central nervous system malignancy. The possibility of multiple sclerosis is not an indication for CSF cytology.
When sending cerebrospinal fluid samples for Cytology, please:
All Trust specimens must be requested on Epic and labelled with an Epic label.
HIGH RISK SPECIMENS: Requests must indicate if a specimen is high risk. Please indicate details of the risk, eg: TB, blood-borne virus, etc.
URGENT SPECIMENS: Please label urgent specimens clearly; an explanation as to why the specimen is urgent is helpful. On receipt, laboratory staff will notify the pathologist who will report the specimen as soon as possible.
The Laboratory aims to comply with The Royal College of Pathologists Key Performance Indicator (KPI 6.4) for Cellular Pathology reporting turnaround times with 90% of all cases reported within 10 calendar days.Specimen Labelling Procedure