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CMV Quantitative PCR (Viral Load)

Microbiology


Summary

Nucleic acid amplification assay for the detection and quantification of Cytomegalovirus (CMV) DNA extracted from whole blood.

The assay used detects a specific target in the exon 4 region of the CMV MIEA gene (major immediate early antigen.)

Usage

Diagnosis / Monitoring of CMV infection in immunocompromised / transplant patients

Background

CMV is a double stranded DNA virus of the Herpesvirus family; it is a common infection (seroprevalence indicates 50-60% of adults in the UK have been infected.) CMV infection is typically unnoticed in healthy people, but can be life threatening for immunocompromised or immunosuppressed patients, such as organ transplant recipients.

Specimen

Whole blood (collected in EDTA)

Specimen Container

EDTA (pink top) 5ml

Special Instructions

Please state type of immunosuppression in the clinical details e.g. transplant patient

Urgent Specimen Instructions

Please contact the laboratory to discuss any urgent requests.

Availability

Local test

Turnaround Time

3 days

Reporting

CMV viral loads will be reported in IU/ml and where appropriate a log will be given.

Lower limit of detection 109 IU/ml

Lower limit of quantification 178 IU/ml

(Please note, we no longer report copies/mL – 1 IU/mL equals approximately 1.4 copies/mL)

 

Specimen Labelling Procedure
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