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Eye Fluid, Corneal Scrape & Contact Lens Culture



Culture of Eye Fluid (i.e. Aqueous, Vitreous taps) / Corneal Scrape Plates / Contact Lenses for the isolation and identification of Bacterial & Fungal Pathogens.

Antimicrobial sensitivity testing is performed as appropriate.


To determine the presence of Bacterial and/or Fungal pathogens which may be causing an infection.

Click here for Acanthamoeba PCR & Culture


Eye Fluid (i.e. Aqueous, Vitreous taps)

Corneal Scrape

Contact Lenses

Click here for Eye Swab Culture

Specimen Container

Corneal Scrape Kit – 4x agar plates (Chocolate Agar, Blood Agar, FAA & SABC) & a glass microscope slide (inside plastic slide carrier)

Sterile container, e.g. white top universal pot (see picture)

Contact Len Case

How To Request On EPIC

For electronic requesting using EPIC (RDUH ONLY):

For Eye Fluids:

Search for: ‘Body Fluid MC&S‘ or test code ‘LAB269

    • Select the appropriate fluid type

For Corneal Scrapes:

Search for: ‘Corneal scrape MC&S‘ or test code ‘LAB9237

    • You will be asked on the request ‘Is testing for Acanthamoeba also required?’ – answer yes if it is and follow the instructions.

For Contact Lenses:

Search for: ‘Medical Device MC&S‘ or test code ‘LAB240

    • Select Contact lens as the sample type
    • If Acanthamoeba testing is required please state in clinical details

Special Instructions

Please provide relevant clinical details with the request: this helps the lab process the sample correctly and provide the best result for the patient.

For Hospital use – a stock of corneal scrape kits are kept in the top of the specimen fridge in Pathology reception in the Old Path Lab building.

Corneal scrapes – if requesting Acanthamoeba PCR & Culture, please send scalpel blade with saline in a sterile universal container.


Processed locally in Microbiology at RDUH

Processing Time

48 – 72 Hours (7 days for extended fungal culture)

Specimen Labelling Procedure
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